Composite
TL sensor

Part:BBa_K3773516:Design

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-15)


Circuit to report translation in vivo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
    Illegal SpeI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 868
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This circuit uses the same sequence as provided by Ceroni et al, 20151.

UNS 1 and UNS 10 flank this part in order to allow for easy Gibson assembly as detailed by Torella et al., 20142.


Source

1Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. Nature Methods, 12(5):415-418. DOI: 10.1038/nmeth.3339

2Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2014). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, 42(1), 681-689.

References